Name : Human Factor H/CFH Protein
Product Source :
Recombinant Human Factor H/CFH Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser860-Arg1231.[Accession | P08603-1]
Molecular Weight :
The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Factor H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Factor H is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Factor H, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
Background :
Factor H is the major soluble inhibitor of complement, where its binding to self markers (i.e., particular glycan structures) prevents complement activation and amplification on host surfaces. Not surprisingly, mutations and polymorphisms that affect recognition of self by Factor H are associated with diseases of complement dysregulation, such as age-related macular degeneration and atypical haemolytic uremic syndrome. In addition, pathogens (i.e., non-self) and cancer cells (i.e., altered-self) can hijack Factor H to evade the immune response.
Synonyms :
Complement Factor H; H factor 1; CFH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; FH; FHL1; HUS
References & Citations :
(1) Parente R, Clark SJ, Inforzato A, Day AJ. Complement Factor H in host defense and immune evasion. Cell Mol Life Sci. 2017 May;74(9):1605-1624. doi: 10.1007/s00018-016-2418-4. Epub 2016 Dec 10. PMID: 27942748; PMCID: PMC5378756.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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