Name : Mouse Complement Factor D/CFD Protein

Product Source :
Recombinant Mouse Complement Factor D/CFD Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile26-Ser259.[Accession | P03953-1]

Molecular Weight :
The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Supplied as 0.22μm filtered solution in 20mM Tris,500mM NaCl (pH 7.4).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse Complement Factor D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse Complement Factor D is greater than 95% as determined by SEC-HPLC.

Background :
Complement Factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement Factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.

Synonyms :
Adipsin; C3 convertase activator; Complement Factor D; CFD; PFD; DF; ADN; Factor D; AMBP-1; EC 3.4.21; EC 3.4.21.46

References & Citations :
(1) Abrera-Abeleda MA, Xu Y, Pickering MC, Smith RJ, Sethi S. Mesangial immune complex glomerulonephritis due to complement Factor D deficiency. Kidney Int. 2007 Jun;71(11):1142-7. doi: 10.1038/sj.ki.5002235. Epub 2007 Apr 4. PMID: 17410102.

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