Name : Human CTGF/CCN2 Protein
Product Source :
Recombinant Human CTGF/CCN2 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Gln27-Ala349.[Accession | Q5M8T4]
Molecular Weight :
The protein has a predicted MW of 61.77 kDa. Due to glycosylation, the protein migrates to 50-55 kDa and 62-72 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CTGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CTGF is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CTGF, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CTGF Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
Background :
Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis.CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.
Synonyms :
IGFBP8; IBP-8; CCN2; NOV2; HCS24; CTGF; CTGRP; Fisp12; MGC102839
References & Citations :
(1) Toda N, Mukoyama M, Yanagita M, Yokoi H. CTGF in kidney fibrosis and glomerulonephritis. Inflamm Regen. 2018 Aug 6;38:14. doi: 10.1186/s41232-018-0070-0. PMID: 30123390; PMCID: PMC6091167.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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