Name : Human Clusterin Protein
Product Source :
Recombinant Human Clusterin Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp23-Glu449.[Accession | P10909-1]
Molecular Weight :
The protein has a predicted MW of 51.2 kDa. The protein migrates to 38-41 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Clusterin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human Clusterin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Clusterin Antibody, hFc Tag with the EC50 of 49.2ng/ml determined by ELISA.
Background :
Clusterin (CLU) is a stress-activated, ATP-independent molecular chaperone, normally secreted from cells, that is up-regulated in Alzheimer disease and in many cancers. It plays important roles in protein homeostasis/proteostasis, inhibition of cell death pathways, and modulation of pro-survival signalling and transcriptional networks. Changes in the CLU gene locus are highly associated with Alzheimer disease, and many therapy-resistant cancers over-express CLU.
Synonyms :
Clusterin; Apo-J; Clustrin; SGP-2; Msgp-2; CLU; AAG4; APOJ; CLI; KUB1; SP-40; TRPM2; CLI; KUB1;
References & Citations :
(1)Wilson MR, Zoubeidi A. Clusterin as a therapeutic target. Expert Opin Ther Targets. 2017 Feb;21(2):201-213. doi: 10.1080/14728222.2017.1267142. Epub 2016 Dec 16. PMID: 27978767.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Neurofilament light polypeptide/NEFLStorage & Stability
ADAM12 ProteinBiological Activity
Popular categories:
CEA Cell Adhesion Molecule 19 (CEACAM19)
Oxidative Stress Responsive Kinase 1 (OXSR1)