Name : Human G-CSF Protein
Product Source :
Recombinant Human G-CSF Protein is expressed from HEK293 with hFc tag at the N-terminus. It contains Thr31-Pro204.[Accession | NP_757373.1]
Molecular Weight :
The protein has a predicted MW of 44.20 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human G-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human G-CSF is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human G-CSF R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Background :
Granulocyte colony-stimulating factor (G-CSF) is an hemopoietic growth factor produced by fibroblasts, monocytes and endothelial cells. G-CSF is expressed by numerous cell types including neuronal, immune and endothelial cells. G-CSF is released in autocrine manner and binds to its receptor G-CSF-R which further activates numerous signaling transduction pathways including PI3K/AKT, JAK/STAT and MAP kinase, and thereby promote neuronal survival, proliferation, differentiation, mobilization of hematopoietic stem and progenitor cells.
Synonyms :
CSF3; GCSF; C17orf33; CSF3OS;
References & Citations :
(1) Visani G, Manfroi S. G-CSF in the biology and treatment of acute myeloid leukemias. Leuk Lymphoma. 1995 Aug;18(5-6):423-8. doi: 10.3109/10428199509059640. PMID: 8528048.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CD79B Proteinmedchemexpress
CD28 ProteinBiological Activity
Popular categories:
Siglec
Serpinb3a