Name : Human M-CSF/CSF-1 Protein
Product Source :
Recombinant Human M-CSF/CSF-1 Protein is expressed from HEK293 without tag. It contains Glu33-Asn190.[Accession | P09603-1]
Molecular Weight :
The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human M-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human M-CSF at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8ng/ml determined by ELISA (QC Test). SPR Data Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200). Cell Based Assay Measured in a cell proliferation assay using RAW264.7 cells.The ED50 for this effect is 2.5 – 7.0 ng/mL (QC Test).
Background :
CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Synonyms :
CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim
References & Citations :
(1)Cannarile M A , Weisser M , Jacob W , et al. Colony-stimulating factor 1 receptor (CSF1R) inhibitors in cancer therapy[J]. Journal for ImmunoTherapy of Cancer, 2017, 5(1):53.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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