Name : Cynomolgus FAP Protein
Product Source :
Recombinant Cynomolgus FAP Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Leu26-Asp760.[Accession | XP_005573377]
Molecular Weight :
The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus FAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus FAP is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus FAP at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test). Bioactivity Data Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2500 pmol/min/µg (QC Test).
Background :
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Synonyms :
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
References & Citations :
(1)Zou B, Liu X, Zhang B, et al. The Expression of FAP in Hepatocellular Carcinoma Cells is Induced by Hypoxia and Correlates with Poor Clinical Outcomes. J Cancer. 2018;9(18):3278-3286. Published 2018 Sep 7. doi:10.7150/jca.25775.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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