Name : Human Fas/TNFRSF6/CD95 Protein
Product Source :
Recombinant Human Fas/TNFRSF6/CD95 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln26-Asn173.[Accession | P25445-1]
Molecular Weight :
The protein has a predicted MW of 13.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Fas on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SPR Data Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
Background :
CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE).
Synonyms :
FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR
References & Citations :
(1)Guégan JP, Legembre P. Nonapoptotic functions of Fas/CD95 in the immune response. FEBS J. 2018 Mar;285(5):809-827. doi: 10.1111/febs.14292. Epub 2017 Nov 16. PMID: 29032605.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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