Name : Human Fas/TNFRSF6/CD95 Protein
Product Source :
Recombinant Human Fas/TNFRSF6/CD95 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln26-Asn173.[Accession | P25445-1]
Molecular Weight :
The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Fas on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Fas is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA.
Background :
CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE).
Synonyms :
FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR
References & Citations :
(1)Guégan JP, Legembre P. Nonapoptotic functions of Fas/CD95 in the immune response. FEBS J. 2018 Mar;285(5):809-827. doi: 10.1111/febs.14292. Epub 2017 Nov 16. PMID: 29032605.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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