Name : Human ficolin 1/FCN1 Protein
Product Source :
Recombinant Human ficolin 1/FCN1 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ala30-Ala326.[Accession | NP_001994.2]
Molecular Weight :
The protein has a predicted MW of 33.11 kDa. Due to glycosylation, the protein migrates to 34-38 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS, 350mM NaCl, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ficolin 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
The ficolins are members of a recently discovered family of host innate opsonins that can activate the lectin pathway of complement. The ficolins bind many ligands, although they are typically described as binding acetylated sugars. Ficolin-1 is the least-described of the Ficolins and is expressed by monocytes, granulocytes, and in the lungs. Ficolin-1 is found circulating at low concentrations in serum but is regarded primarily as a secretory molecule that exerts its function locally in inflamed tissues.
Synonyms :
ficolin 1; FCN1; ficolin1; Ficolin-A; M-ficolin; FCNM
References & Citations :
(1) Brady AM, Spencer BL, Falsey AR, Nahm MH. Blood collection tubes influence serum ficolin-1 and ficolin-2 levels. Clin Vaccine Immunol. 2014 Jan;21(1):51-5. doi: 10.1128/CVI.00607-13. Epub 2013 Oct 30. PMID: 24173025; PMCID: PMC3910923.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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