Name : Human FCRL2/IRTA4 Protein
Product Source :
Recombinant Human FCRL2/IRTA4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu20-Val401.[Accession | Q96LA5-1]
Molecular Weight :
The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FCRL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human FCRL2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FCRL2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FCRL2 Antibody, hFc Tag with the EC50 of 0.13μg/ml determined by ELISA.
Background :
Fc receptor-like 2 (FCRL2) is highly expressed on B-cell chronic lymphocytic leukemia (B-CLL) cells and could possibly influence disease pathogenesis. FCRL2 mRNA expression was found to be expressed at considerably higher levels in peripheral blood mononuclear cells (PBMC) of B-CLL patients compared to controls (range 1.35- to 210-fold upregulation; P < 0.0001) and cells of other hematological diseases.
Synonyms :
FcR-like protein 2; FcRL2; FCRH2; IFGP4; IRTA4; FCRL2; CD307b; FCRH2SPAP1B; IRTA4SPAP1C; SPAP1; SPAP1A
References & Citations :
(1) Nückel H, Collins CH, Frey UH, Sellmann L, Dürig J, Siffert W, Dührsen U. FCRL2 mRNA expression is inversely associated with clinical progression in chronic lymphocytic leukemia. Eur J Haematol. 2009 Dec 1;83(6):541-9. doi: 10.1111/j.1600-0609.2009.01328.x. Epub 2009 Aug 4. PMID: 19682311.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations: Zika virus E/Envelope Protein (HEK293 HMGB1/HMG-1 Protein C1-Inhibitor Integrin alpha X