Name : Biotinylated Human FGFR3 beta (IIIb) Protein
Product Source :
Recombinant Biotinylated Human FGFR3 beta (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp127-Gly377.[Accession | P22607-2]
Molecular Weight :
The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human FGFR3 beta (IIIb) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human FGFR3 beta (IIIb) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human FGFR3 beta (IIIb) , His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
Background :
Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis.
Synonyms :
ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4
References & Citations :
(1)Hadari Y , Schlessinger J. FGFR3-targeted mAb therapy for bladder cancer and multiple myeloma[J]. The Journal of clinical investigation, 2009, 119(5):1077-1079.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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