Name : Biotinylated Human FGL1 Protein (Primary Amine Labeling)
Product Source :
Recombinant Biotinylated Human FGL1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus. It contains Asp64-Asn305.[Accession | Q08830]
Molecular Weight :
The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in 20mM PB, 250mM NaCl (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human FGL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human FGL1 is greater than 90% as determined by SEC-HPLC. ELISA Data Immobilized Human LAG3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Background :
Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Synonyms :
FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand
References & Citations :
(1)Wang, J., Sanmamed, M.F., Datar, I., Su, T.T., Ji, L., Sun, J., Chen, L., Chen, Y., Zhu, G., Yin, W., et al. (2019). Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3. Cell 176, 334-347.e312.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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