Name : Biotinylated Mouse APLN Protein
Product Source :
Recombinant Biotinylated Mouse APLN Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Val23-Phe77.[Accession | Q9R0R4]
Molecular Weight :
The protein has a predicted MW of 34.81 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 0.2M Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Mouse APLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages.
Synonyms :
Apelin;APJ endogenous ligand;APEL
References & Citations :
(1) Arababadi MK, Asadikaram P, Asadikaram G. APLN/APJ pathway: The key regulator of macrophage functions. Life Sci. 2019 Sep 1;232:116645. doi: 10.1016/j.lfs.2019.116645. Epub 2019 Jul 10. PMID: 31299236.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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