Name : Human NKG2D/CD314 Protein
Product Source :
Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus. It contains Phe78-Val216.[Accession | P26718]
Molecular Weight :
The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ULBP-2, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 2.8μg/ml determined by ELISA (QC Test). ELISA Data Immobilized Human MICA, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.88μg/ml determined by ELISA. Blocking Data Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
Background :
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Synonyms :
CD314; D12S2489E; KLR; NKG2-D; NKG2D
References & Citations :
(1)Spear P , Barber A , Rynda-Apple A , et al. NKG2D CAR T-cell therapy inhibits the growth of NKG2D ligand heterogeneous tumors[J]. Immunology and Cell Biology, 2013, 91(6):435-440.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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