Name : Biotinylated Human NKp30/NCR3/CD337 Protein
Product Source :
Recombinant Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu19-Thr138.[Accession | AAH52582]
Molecular Weight :
The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human NKp30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human NKp30 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human NKp30, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). SPR Data Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NKp30, His-Avi Tag with an affinity constant of 0.330 μM as determined in SPR assay (Biacore T200).
Background :
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors”. These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells.
Synonyms :
CD337; LY117; NCR3; NKp30; 1C7; MALS
References & Citations :
(1) Strandmann E P V , Shatnyeva O , Hansen H P. NKp30 and its ligands: Emerging players in tumor immune evasion from natural killer cells[J]. Annals of Translational Medicine, 2015.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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