Name : Human NPC2 Protein
Product Source :
Recombinant Human NPC2 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Glu20-Leu151.[Accession | P61916-1]
Molecular Weight :
The protein has a predicted MW of 15.67 kDa. Due to glycosylation, the protein migrates to 21-24 kDa and 27-35 kDa kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human NPC2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
The Niemann Pick type C (NPC) proteins, NPC1 and NPC2, are involved in the lysosomal storage disease, NPC disease. The formation of a NPC1⁻NPC2 protein⁻protein complex is believed to be necessary for the transfer of cholesterol and lipids out of the late endosomal (LE)/lysosomal (Lys) compartments. Mutations in either NPC1 or NPC2 can lead to an accumulation of cholesterol and lipids in the LE/Lys, the primary phenotype of the NPC disease.
Synonyms :
He1; EDDM1
References & Citations :
Hodošček M, Elghobashi-Meinhardt N. Simulations of NPC1(NTD):NPC2 Protein Complex Reveal Cholesterol Transfer Pathways. Int J Mol Sci. 2018 Sep 4;19(9):2623. doi: 10.3390/ijms19092623. PMID: 30181526; PMCID: PMC6163316.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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