Name : Biotinylated Human uPAR/PLAUR Protein
Product Source :
Recombinant Biotinylated Human uPAR/PLAUR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu23-Gly305.[Accession | Q03405-1]
Molecular Weight :
The protein has a predicted MW of 34.36 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human uPAR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human uPAR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human PLAU, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human uPAR, His Tag with the EC50 of 13.4ng/ml determined by ELISA.
Background :
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
Synonyms :
U-PAR; uPAR; CD87; PLAUR; MO3; UPAR
References & Citations :
(1) Blasi F. The urokinase receptor and cell migration. Semin Thromb Hemost. 1996;22(6):513-6. doi: 10.1055/s-2007-999053. PMID: 9122717.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
BSP2 Protein
Angiopoietin-2 Protein
Popular categories:
4-1BB/CD137
CD49f/Integrin alpha-6