Name : Biotinylated Human PDGF R alpha/PDGFRA Protein
Product Source :
Recombinant Biotinylated Human PDGF R alpha/PDGFRA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln24-Glu524.[Accession | P16234]
Molecular Weight :
The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human PDGF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human PDGF R alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-PDGF R alpha Antibody, hFc Tag T at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human PDGF R alpha, His Tag with the EC50 of 0.16μg/ml determined by ELISA.
Background :
Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.
Synonyms :
PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2
References & Citations :
(1)Shi Y, He G, Lee WC, McKenzie JA, Silva MJ, Long F. Gli1 identifies osteogenic progenitors for bone formation and fracture repair. Nat Commun. 2017;8(1):2043. Published 2017 Dec 11. doi:10.1038/s41467-017-02171-2
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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