Name : Cynomolgus PDGF R beta/CD140b Protein
Product Source :
Recombinant Cynomolgus PDGF R beta/CD140b Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu33-Lys531.[Accession | A0A2K5TUS0]
Molecular Weight :
The protein has a predicted MW of 57.49 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus PDGF R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus PDGF R beta is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus PDGFR beta, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-PDGFRB Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Background :
Platelet-derived growth factor receptor (PDGFR) signaling is involved in proliferation and survival in a wide array of cell types.PDGFR-β signalling, via TGF-β signalling, may be crucial for restoration of BBB integrity after cerebral ischemia and therefore represents a novel potential therapeutic target.
Synonyms :
PDGF-R-beta; PDGFR-beta; PDGFRB; PDGF R beta; PDGFR-2; PDGFR-β
References & Citations :
(1)Yue Z, Chen J, Lian H, Pei J, Li Y, Chen X, Song S, Xia J, Zhou B, Feng J, Zhang X, Hu S, Nie Y. PDGFR-β Signaling Regulates Cardiomyocyte Proliferation and Myocardial Regeneration. Cell Rep. 2019 Jul 23;28(4):966-978.e4. doi: 10.1016/j.celrep.2019.06.065. PMID: 31340157.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
KCIP-1 Protein
GFPT1 Protein
Popular categories:
DNGR-1/CLEC9A
Lymphocyte Function Associated Antigen 1 (LFA-1)