Name : Cynomolgus CDCP1 Protein
Product Source :
Recombinant Cynomolgus CDCP1 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Phe30-Thr667.[Accession | XP_005546930.2]
Molecular Weight :
The protein has a predicted MW of 73.15 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus CDCP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus CDCP1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus CDCP1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
Background :
Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
Synonyms :
CDCP1; CD318; SIMA135; TRASK;
References & Citations :
(1)Wright HJ, Hou J, Xu B, Cortez M, Potma EO, Tromberg BJ, Razorenova OV. CDCP1 drives triple-negative breast cancer metastasis through reduction of lipid-droplet abundance and stimulation of fatty acid oxidation. Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):E6556-E6565. doi: 10.1073/pnas.1703791114. Epub 2017 Jul 24. PMID: 28739932; PMCID: PMC5559020.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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