Name : Human Annexin V/ANXA5 Protein
Product Source :
Recombinant Human Annexin V/ANXA5 Protein is expressed from E.coli without tag. It contains Ala2-Asp320.[Accession | P08758]
Molecular Weight :
The protein has a predicted MW of 35.9 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in 50mM Tris, 240mM NaCl (pH 8.5).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Annexin V/ANXA5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Annexin V/ANXA5 is greater than 95% as determined by SEC-HPLC.
Background :
Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.
Synonyms :
Annexin A5; Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; PP4; PAP-I; VAC-alpha; ANX5; ENX2; ANXA5; HEL-S-7; RPRGL3
References & Citations :
(1) Rieger AM, Nelson KL, Konowalchuk JD, Barreda DR. Modified annexin V/propidium iodide apoptosis assay for accurate assessment of cell death. J Vis Exp. 2011 Apr 24;(50):2597. doi: 10.3791/2597. PMID: 21540825; PMCID: PMC3169266.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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