Name : Human CLEC2D Protein

Product Source :
Recombinant Human CLEC2D Protein is expressed from HEK293 with hFc tag and Avi tag at the N-Terminus. It contains Arg60-Val191.[Accession | Q9UHP7-1]

Molecular Weight :
The protein has a predicted MW of 59.1 kDa. Due to glycosylation, the protein migrates to 72-78 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human CLEC2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CLEC2D is greater than 95% as determined by SEC-HPLC.

Background :
C-type lectin domain family 2, member D (CLEC2D) is implicated in the immune response. Pre-eclampsia and HIV infection have opposing immune responses. The contrasting expression of CLEC2D in HIV infection and pre-eclampsia is demonstrative of the immunosuppressive and pro-inflammatory roles of the respective pathologies. However, this implication may be confounded by highly active anti-retroviral treatment (HAART).

Synonyms :
C-type lectin domain family 2 member D;Lectin-like NK cell receptor;Lectin-like transcript 1;LLT-1;CLAX;LLT1;OCIL;CLEC2D

References & Citations :
(1) Varaden D, Moodley J, Onyangunga OA, Naicker T. Morphometric image analysis of placental C-type lectin domain family 2, member D (CLEC2D) immuno-expression in HIV associated pre-eclampsia. Eur J Obstet Gynecol Reprod Biol X. 2019 May 8;3:100039. doi: 10.1016/j.eurox.2019.100039. PMID: 31403127; PMCID: PMC6687384.

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