Name : Mouse CPM Protein
Product Source :
Recombinant Mouse CPM Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu18-Ser423.[Accession | Q80V42-1]
Molecular Weight :
The protein has a predicted MW of 47.51 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse CPM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CPM is greater than 95% as determined by SEC-HPLC.
Background :
Carboxypeptidase M (CPM) is a glycosylphosphatidylinositol anchored enzyme that plays an important role in the kallikrein-kinin system (KKS). CPM catalytic domain hydrolyzes Arg from C-terminal peptides (i.e., bradykinin and kallidin), generating des-Arg-kinins, the agonists of B1 receptor (B1R).
Synonyms :
Carboxypeptidase M; 1110060I01Rik; AA589379
References & Citations :
Guimarães PB, da Silva RF, Hoff CC, Fernandes L, Nakaie CR, Chagas JR, Carmona AK, Bader M, Pesquero JB. Interactions between Carboxypeptidase M and kinin B1 receptor in endothelial cells. Inflamm Res. 2019 Oct;68(10):845-855. doi: 10.1007/s00011-019-01264-6. Epub 2019 Jun 19. PMID: 31218444.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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