Name : Cynomolgus CRTAM Protein
Product Source :
Recombinant Cynomolgus CRTAM Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser18-Gly287.[Accession | XP_005580021.1]
Molecular Weight :
The protein has a predicted MW of 30.80 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus CRTAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Cynomolgus CRTAM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA.
Background :
Class-I Restricted T Cell-Associated Molecule (CRTAM) is a protein that is expressed after T cell activation. The interaction of CRTAM with its ligand, nectin-like 2 (Necl2), is required for the efficient production of IL-17, IL-22, and IFNγ by murine CD4 T cells, and it plays a role in optimal CD8 T and NK cell cytotoxicity. CRTAM promotes the pro-inflammatory cytokine profile; therefore, it may take part in the immunopathology of autoimmune diseases such as diabetes type 1 or colitis.
Synonyms :
CD355 antigen; CD355; CRTAM
References & Citations :
(1) Takeuchi A, Itoh Y, Takumi A, Ishihara C, Arase N, Yokosuka T, Koseki H, Yamasaki S, Takai Y, Miyoshi J, Ogasawara K, Saito T. CRTAM confers late-stage activation of CD8 T cells to regulate retention within lymph node. J Immunol. 2009 Oct 1;183(7):4220-8. doi: 10.4049/jimmunol.0901248. Epub 2009 Sep 14. PMID: 19752223.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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