Name : Biotinylated Cynomolgus FAP Protein
Product Source :
Recombinant Biotinylated Cynomolgus FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Leu26-Asp760.[Accession | XP_005573377]
Molecular Weight :
The protein has a predicted MW of 88 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 200mM Arginine (pH 8.2).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Cynomolgus FAP on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Cynomolgus FAP is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-FAP Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus FAP, His Tag with the EC50 of 58.5ng/ml determined by ELISA.
Background :
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Synonyms :
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
References & Citations :
(1)Zou B, Liu X, Zhang B, et al. The Expression of FAP in Hepatocellular Carcinoma Cells is Induced by Hypoxia and Correlates with Poor Clinical Outcomes. J Cancer. 2018;9(18):3278-3286. Published 2018 Sep 7. doi:10.7150/jca.25775.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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