Rabbit anti-p63 Polyclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description p63 Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of TP53/p53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. |Synonym Chronic ulcerative stomatitis protein (CUSP); Keratinocyte transcription factor KET; Transformation-related protein 63 (TP63); Tumor protein p73-like (p73L); p40; p51; KET; P63; P73H; P73L; TP73L |Host Rabbit |Specificity p63 antibody detects endogenous levels of total p63. |Species Reactivity Human, Mouse |Applications WB: 1:500~1:3000, IF/ICC: 1:100~1:500, ELISA(peptide) 1:20000-1:40000 |Immunogen A synthesized peptide derived from Human TP63(Accession Q9H3D4), corresponding to amino acid residues P654-E678. |Properties |Form liquid |Concentration 1mg/ml |Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |Clonality Monoclonal antibody |Clone 054-38 |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |Target |Background Tumor protein p63, typically referred to as p63, also known as transformation-related protein 63 is a protein that in humans is encoded by the TP63 (also known as the p63) gene. Transcription factor p63 is a key regulator of epidermal keratinocyte proliferation and differentiation. Tumor protein p63 is a member of the p53 family of transcription factors. p63 regulates the activity of a multitude of genes involved in growth and development of the ectoderm and derived structures and tissues, such as basal layer keratins and cell cycle control genes. Accordingly, p63 expression is found in basal cell layers of various organs, squamous epithelial cells of many organs and urothelium. p63 (TAp63) is closely related to p40 (ΔNp63) as both proteins represent isoforms of the p63 gene with distinct molecular functions. While “full length” p63 (TAp63) activates p53 target genes such as p21 or BAX, the shorter transcript p40 (ΔNp63) inhibits activation of p53 and “full length” p63. P63 is also helpful in distinguishing poorly differentiated squamous cell carcinoma from small cell carcinoma or adenocarcinoma. P63 should be strongly stained in poorly differentiated squamous cell, but negative in small cell or adenocarcinoma. |tissuespecificity Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. |Post-translational modifications May be sumoylated.Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. |Cellular localization Cytosol;Endoplasmic reticulum;Golgi apparatus;Mitochondrion;Nucleus; |UniProt Q9H3D4 | Western blot analysis on HeLa cell lysate using p63 Antibody |Tips:This product is for research use only. Not for use in diagnostic prodcedures.